Dose-dependency of a combined EPA:DHA mixture on incorporation, washout, and protein synthesis in C2C12 myotubes

M Banic; M van Dijk; F J Dijk; M J W Furber; O C Witard; N Donker; M J A Becker; S D Galloway; N Rodriguez-Sanchez

doi:10.1016/j.plefa.2024.102651

Dose-dependency of a combined EPA:DHA mixture on incorporation, washout, and protein synthesis in C2C12 myotubes

Prostaglandins Leukot Essent Fatty Acids. 2024 Sep 25:203:102651. doi: 10.1016/j.plefa.2024.102651. Online ahead of print.

Authors

M Banic¹, M van Dijk², F J Dijk², M J W Furber², O C Witard³, N Donker², M J A Becker², S D Galloway⁴, N Rodriguez-Sanchez⁴

Affiliations

¹ University of Stirling, Physiology, Exercise and Nutrition Research Group, Faculty of Health Sciences and Sport, Stirling, United Kingdom. Electronic address: [email protected].
² Danone Global Research & Innovation Center B.V., Utrecht, the Netherlands.
³ King's College London, Centre for Human and Applied Physiological Sciences, School of Basic & Medical Biosciences, Faculty of Life Sciences & Medicine, London, United Kingdom.
⁴ University of Stirling, Physiology, Exercise and Nutrition Research Group, Faculty of Health Sciences and Sport, Stirling, United Kingdom.

PMID: 39541834
DOI: 10.1016/j.plefa.2024.102651

Abstract

We demonstrate divergent incorporation and washout patterns for EPA and DHA following high and low-dose EPA+DHA incubation in C2C12 myotubes, with higher concentrations favoring n-3 PUFA incorporation. Lower n-3 PUFA concentrations increased MPS without further upregulating the mTORC1 signaling pathway. Our study provides novel insights into the temporal incorporation and washout dynamics of EPA and DHA and, specifically, their combined effect on MPS, thereby advancing knowledge regarding dietary n-3 PUFA prescription to promote skeletal muscle health in humans.

Keywords: Dose; Fish oil; Muscle; Time-course; mTORC1 signaling; n-3 PUFA.