Deciphering the molecular mechanism underlying morphology transition in two-component DNA-protein cophase separation

Structure. 2024 Nov 5:S0969-2126(24)00461-1. doi: 10.1016/j.str.2024.10.026. Online ahead of print.

Abstract

Nucleic acid and protein co-condensates exhibit diverse morphologies crucial for fundamental cellular processes. Despite many previous studies that advanced our understanding of this topic, several interesting biophysical questions regarding the underlying molecular mechanisms remain. We investigated DNA and human transcription factor p53 co-condensates-a scenario where neither dsDNA nor the protein demonstrates phase-separation behavior individually. Through a combination of experimental assays and theoretical approaches, we elucidated: (1) the phase diagram of DNA-protein co-condensates at a certain observation time, identifying a phase transition between viscoelastic fluid and viscoelastic solid states, and a morphology transition from droplet-like to "pearl chain"-like co-condensates; (2) the growth dynamics of co-condensates. Droplet-like and "pearl chain"-like co-condensates share a common initial critical microscopic cluster size at the nanometer scale during the early stage of phase separation. These findings provide important insights into the biophysical mechanisms underlying multi-component phase separation within cellular environments.

Keywords: AFM-FS; CMC; DNA-protein interactive co-condensate; DPIC; VPS; atomic force microscopy-based force spectroscopy; biomolecular condensate; coarse-grained molecular dynamics; critical microscopic cluster; dcFCCS; dual-color fluorescence cross-correlation spectroscopy; growth dynamics of co-condensate; morphology regulation; multicomponent phase diagram; viscoelastic phase separation.