Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides

Guangzheng Sun; Yeqiang Xia; Kuikui Li; Qinsheng Zhu; Feifei Ding; Hui Gu; Zhichao Zhang; Xinrui Li; Xuan Mi; Jun Chen; Ruoting Yao; Sicong Zhang; Haibing Ouyang; Xi Chen; Tengfei Liu; Haibin Jiang; Yao Zhao; Min Qiu; Wenwu Ye; Kaixuan Duan; Zhenchuan Ma; Suomeng Dong; Heng Yin; Yan Wang; Yuanchao Wang

doi:10.1007/s11427-024-2724-5

Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides

Sci China Life Sci. 2024 Nov 13. doi: 10.1007/s11427-024-2724-5. Online ahead of print.

Authors

Guangzheng Sun^#^{1

2

3}, Yeqiang Xia^#^{1

2}, Kuikui Li⁴, Qinsheng Zhu^{1

2}, Feifei Ding^{1

2}, Hui Gu⁴, Zhichao Zhang^{1

2}, Xinrui Li^{1

2}, Xuan Mi^{1

2}, Jun Chen^{1

2}, Ruoting Yao^{1

2}, Sicong Zhang^{1

2}, Haibing Ouyang^{1

2}, Xi Chen^{1

2}, Tengfei Liu^{1

2}, Haibin Jiang^{1

2}, Yao Zhao^{1

2

3}, Min Qiu^{1

2}, Wenwu Ye^{1

2}, Kaixuan Duan^{1

2}, Zhenchuan Ma^{1

2}, Suomeng Dong^{1

2}, Heng Yin⁵, Yan Wang^{6

7}, Yuanchao Wang^{8

9}

Affiliations

¹ Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
² The Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing, 210095, China.
³ Suzhou Academy of Agricultural Sciences, Suzhou, 234000, China.
⁴ Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Technology Innovation Center for Green Agriculture, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
⁵ Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Technology Innovation Center for Green Agriculture, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. [email protected].
⁶ Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China. [email protected].
⁷ The Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing, 210095, China. [email protected].
⁸ Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China. [email protected].
⁹ The Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing, 210095, China. [email protected].

^# Contributed equally.

PMID: 39549112
DOI: 10.1007/s11427-024-2724-5

Abstract

Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity. Herein, we identified a polysaccharide lyase 1 protein, PsPL1, which acts as an essential virulence factor of P. sojae infection in soybean. However, the overexpression of PsPL1 in P. sojae reduced infection and triggered enhanced immune responses in soybean. PsPL1 exhibited pectin lyase activity and degraded plant pectin to generate pectin oligosaccharides (POSs) with a polymerization degree of 3-14, exhibiting different levels of acetylation and methylation modifications. PsPL1 and the degraded pectin products triggered immune responses in soybean and different Solanaceous plants. The PsPL1-triggered immune responses required RSPL1, a membrane-localized leucine-rich repeat receptor-like protein, which is essential for Phytophthora resistance. Conversely, the PsPL1-degraded product-triggered immune responses depended on the membrane-localized lysin motif receptor-like kinase CERK1. This study reveals that the pectin lyase exhibits a dual immunogenic role during P. sojae infection, which activates plant resistance through different immune receptors and provides novel insights into the function of pectin lyase in host-pathogen interactions.

Keywords: Phytophthora sojae; immune activation; pathogenicity; pectin lyase; pectin oligosaccharide; recognition receptor.