Protocol for generation of PD-modeling-induced neurons and detection of α-synuclein forms

Francesco V Nardi; Gulimiheranmu Maisumu; You Zhou; Bo Liang; Abraam M Yakoub

doi:10.1016/j.xpro.2024.103447

Protocol for generation of PD-modeling-induced neurons and detection of α-synuclein forms

STAR Protoc. 2024 Nov 15;5(4):103447. doi: 10.1016/j.xpro.2024.103447. Online ahead of print.

Authors

Francesco V Nardi¹, Gulimiheranmu Maisumu¹, You Zhou², Bo Liang³, Abraam M Yakoub⁴

Affiliations

¹ Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA 02115, USA; Biomedical Engineering Program, College of Engineering and Mines, University of North Dakota, Grand Forks, ND 58202, USA.
² Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA 02115, USA.
³ Biomedical Engineering Program, College of Engineering and Mines, University of North Dakota, Grand Forks, ND 58202, USA.
⁴ Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA 02115, USA. Electronic address: [email protected].

PMID: 39549235
DOI: 10.1016/j.xpro.2024.103447

Abstract

Alpha-synuclein (α-Syn) is an important molecule in the pathogenesis of Parkinson's and Alzheimer's disease-related dementias such as Lewy body dementia, forming multiple pathological species. In vitro disease models, including human neurons and α-Syn-transfected cells, are instrumental to understand synucleinopathies or test new therapies. Here, we provide a detailed protocol to generate human neurons derived from induced pluripotent stem cells (iPSCs), and HEK cells, with α-Syn mutations. We also describe multiple assays to determine the various α-Syn forms.

Keywords: Biotechnology and bioengineering; Molecular Biology; Neuroscience; Stem Cells.