Generation of TWO iPSC lines (CRICKi009-A; CRICKi010-A) from patients with type 1 von Hippel-Lindau (VHL) and histopathologically confirmed renal cell carcinoma (RCC)

Liani G Devito; Eugénie S Lim; Samuel M O'Toole; Scott T C Shepherd; Daqi Deng; Hugang Feng; Taja Barber; William M Drake; Samra Turajlic; Lyn Healy

doi:10.1016/j.scr.2024.103611

Generation of TWO iPSC lines (CRICKi009-A; CRICKi010-A) from patients with type 1 von Hippel-Lindau (VHL) and histopathologically confirmed renal cell carcinoma (RCC)

Stem Cell Res. 2024 Nov 14:81:103611. doi: 10.1016/j.scr.2024.103611. Online ahead of print.

Authors

Affiliations

¹ Human Embryo and Stem Cell Unit, The Francis Crick Institute, London, UK.
² Department of Endocrinology, St. Bartholomew's Hospital, Barts Health NHS Trust, London, UK.
³ Cancer Dynamics Laboratory, The Francis Crick Institute, London, UK; Skin and Renal Units, The Royal Marsden NHS Foundation Trust, London, UK; Melanoma and Kidney Cancer Team, The Institute of Cancer Research, London, UK.
⁴ Cancer Dynamics Laboratory, The Francis Crick Institute, London, UK.
⁵ Cancer Dynamics Laboratory, The Francis Crick Institute, London, UK; Skin and Renal Units, The Royal Marsden NHS Foundation Trust, London, UK; Melanoma and Kidney Cancer Team, The Institute of Cancer Research, London, UK. Electronic address: [email protected].
⁶ Human Embryo and Stem Cell Unit, The Francis Crick Institute, London, UK. Electronic address: [email protected].

PMID: 39549334
DOI: 10.1016/j.scr.2024.103611

Abstract

VHL disease is an inherited and autosomal dominant disorder affecting 1 in 36,0000 individuals worldwide. It is caused by von Hippel-Lindau (VHL) gene mutations and can affect both genders and all ethnic backgrounds (Nordstrom-O'Brien et al., 2009; Maher, 2004). Here, we generated and characterised two iPSC lines derived from patients with histopathologically confirmed clear cell renal cell carcinoma (ccRCC) and VHL Type 1 enrolled in the TRACERx Renal (TRAcking Renal Cell Carcinoma Evolution Through Therapy (Rx)). PBMCs were reprogrammed to pluripotency using a genome non-integrating Sendai virus (SeV) vectors protocol. Both human iPSC lines displayed normal morphology, expressed markers associated with stemness and differentiated into the three germ layers. The iPSC lines could be used as a disease-specific cellular model to understand furtherthe inherited disorder of Type 1 von Hippel-Lindau (VHL) disease.