Study on the Role of Dnmt3a Expression in the Dentate Gyrus of the Hippocampus in Reward Memory

Objective: Emotional memory has been associated with many psychiatric diseases. Understanding emotional memory could be beneficial in comprehending and discovering new therapies for diseases related to emotional memory, such as depression and post-traumatic stress disorder (PTSD). Our previous study revealed that Dnmt3a expression in the dentate gyrus (DG) contributes to fear memory. However, is there a correlation between Dnmt3a expression in the DG and reward memory? This study aims to explore the relationship between Dnmt3a expression and reward memory.

Methods: We induced fear memory (Fear group) or reward memory (Reward group) using fear conditioning and social interaction in females, respectively. We then measured the expression levels of Dnmt3a and c-fos after the retrieval of different types of memory. Additionally, we used a recombinant Adeno-Associated Virus (rAAV) to overexpress Dnmt3a in the DG and conducted conditioned place preference (CPP) tests to assess changes in reward memory.

Results: We observed a significant increase in Dnmt3a and c-fos expression in the Fear group compared with the Reward group. Overexpression of Dnmt3a in the DG led to an increase in time spent in the white box during CPP tests.

Conclusion: Dnmt3a expression levels varied after the retrieval of fear or reward memory, and overexpression of Dnmt3a in the DG enhanced reward memory. These findings suggest that Dnmt3a expression in the DG plays a role in reward memory.