Reductive soil disinfestation influences microbial aging of low-density polyethylene and polyhydroxyalkanoate microplastics and microbial communities in plastispheres

The extensive use of plastic products has led to the accumulation of microplastics (MPs) in agricultural soils, raising concerns about their fate in various environments. Reductive soil disinfestation (RSD) treatment is increasingly being adopted in various countries to address agricultural soil health issues. However, the treatment can alter the soil microbial environment, potentially affecting the fate of contaminants, including MPs. The effect of RSD on the aging of low-density polyethylene (LDPE) and polyhydroxyalkanoates (PHA) MPs was studied through an incubation experiment. The mechanism involved was further investigated by microbial community analysis. The characterization results shown that RSD treatment inhibited the aging of LDPE but promoted the aging of PHA. The results indicated that RSD reshaped the microbial community and reduced the relative abundance of lipid metabolism in the LDPE plastisphere, thereby hindering LDPE aging. Predicted functional genes in the plastispheres were primarily involved in metabolism (77.15-87.48%) and genetic information processing (8.774-12.62%). The enrichment of bacteria related to poly(3-hydroxybutyrate) depolymerase (phaZ) in the PHA plastisphere explained the higher aging degree of PHA during RSD. Some fungus also involved in the MPs aging, while some fungus pathogens can proliferate in the MPs plastispheres. The 3DEEM analysis indicated that PHA MPs aging increased tyrosine-like substances in soil extracts. These findings provide new insights into the ecological implications of RSD and enhance our understanding of microbial communities within plastispheres.