We report here the discovery and characterization of three novel bacteriophages infecting Mycobacterium smegmatis . These siphoviruses were isolated from soil collected in urban areas around Saint Joseph's University in Philadelphia. Mycobacteriphages Idergollasper, FoulBall, and Schuy are assigned to actinobacteriophage cluster O based on gene content similarity, and have prolate capsids typical for this cluster.
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