A Transgenic Method to Measure Mitotic Exit in Drosophila Embryos

Shige Yamada; Aaron N Johnson; Shuo Yang

doi:10.1007/978-1-0716-4236-8_1

A Transgenic Method to Measure Mitotic Exit in Drosophila Embryos

Methods Mol Biol. 2025:2874:1-8. doi: 10.1007/978-1-0716-4236-8_1.

Authors

Shige Yamada¹, Aaron N Johnson¹, Shuo Yang²

Affiliations

¹ Department of Developmental Biology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
² Department of Genetics and Genetics Engineering, School of Life Science, Fudan University, Shanghai, China. [email protected].

PMID: 39614042
DOI: 10.1007/978-1-0716-4236-8_1

Abstract

Mitotic exit is a necessary step for highly specialized cells to terminally differentiate and acquire unique functions. The FUCCI system can be used to visualize mitotic and post-mitotic cells during development and regeneration in both live organisms and fixed tissues. Here we describe a Fly-FUCCI protocol for assaying mitotic exit in Drosophila embryos.

Keywords: Drosophila embryogenesis; Fly-FUCCI; Mef2; Mitotic exit; Myogenesis.

MeSH terms

Animals
Animals, Genetically Modified
Drosophila / cytology
Drosophila / embryology
Drosophila / genetics
Drosophila melanogaster / cytology
Drosophila melanogaster / embryology
Drosophila melanogaster / genetics
Embryo, Nonmammalian* / cytology
Mitosis*