We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further shielded in an organosilica layer of controlled thickness. Under reducing conditions, disulphide bonds linking the protein to the carrier material were reduced, triggering enzyme activation. The organosilica shield prevents the enzymes from leaching from the nanobiocatalysts and preserves their integrity.
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