(1) Background: Ecotoxicological screening evaluates the acute toxicity of WWs. The Vibrio fischeri ecotoxicological assay analyses inlet and outlet wastewater samples from two urban wastewater treatment plants in Catania, Sicily, Italy. (2) Methods: The APAT CNR IRSA 8030 Man 29 method was used as method; (3) Results: The results showed toxicity values below the limit of the Italian Legislative Decree 152/06; (4) Conclusions: This monitoring study allows to verify the efficacy, and the outlet quality of WWs discharged to sea water. This ecotoxicological assay is a valuable tool for evaluating the combined toxicity of various pollutants that underline the total damage of the studied matrices detecting the true effect of complex mixtures on the environment and its fauna.
Keywords: Bioassay and environmental safeguard; Ecotoxicity; Wastewater.
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