We present a genome assembly from an individual male Myotis mystacinus (whiskered bat; Chordata; Mammalia; Chiroptera; Vespertilionidae). The genome sequence has a total length of 2,081.20 megabases. Most of the assembly (97.52%) is scaffolded into 23 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.93 kilobases in length.
Keywords: Chiroptera; Myotis mystacinus; chromosomal; genome sequence; whiskered bat.
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