Reported prevalence and comparison of diagnostic approaches for Candida africana: a systematic review with meta-analysis

This systematic review and meta-analysis evaluated reported prevalence and diagnostic methods for identifying Candida africana, an opportunistic yeast associated with vaginal and oral candidiasis. A comprehensive literature search yielded 53 studies meeting the inclusion criteria, 2 of which were case studies. The pooled prevalence of C. africana among 20,571 participants was 0.9% (95% CI: 0.7-1.3%), with significant heterogeneity observed (I² = 79%, p < 0.01). Subgroup analyses revealed regional variations, with North America showing the highest prevalence (4.6%, 95% CI: 1.8-11.2%). The majority 84.52% of the C. africana have been isolated from vaginal samples, 8.37% from oral samples, 3.77% from urine, 2.09% from glans penis swabs, and 0.42% from rectal swabs, nasal swabs, and respiratory tract expectorations respectively. No C. africana has been isolated from nail samples. Hyphal wall protein 1 gene PCR was the most used diagnostic method for identifying C. africana. It has been used to identify 70% of the isolates. A comparison of methods revealed that the Vitek-2 system consistently failed to differentiate C. africana from Candida albicans, whereas MALDI-TOF misidentified several isolates compared with HWP1 PCR. Factors beyond diagnostic methodology may influence C. africana detection rates. We highlight the importance of adapting molecular methods for resource-limited settings or developing equally accurate but more accessible alternatives for the identification and differentiation of highly similar and cryptic Candida species such as C. africana.