Correlation between Ki-67 labeling index and mitotic index in Head and neck squamous cell carcinoma

Indian J Pathol Microbiol. 2024 Oct 1;67(4):835-840. doi: 10.4103/ijpm.ijpm_447_23. Epub 2024 Jun 4.

Abstract

Background: Proliferative activity of any tissue or neoplasm can be determined by its growth rate by mitotic count or by using antibodies directed against specific antigens like Ki-67. The usefulness of Ki-67 in head and neck cancers has been debated for the past 20 years; however, no study has definitively resolved this controversy.

Aim: Assess the correlation between Ki-67 expression and mitotic index in head and neck squamous cell carcinoma.

Materials and methods: This cross-sectional study was performed in 66 histologically proven cases of Head and Neck Squamous cell carcinoma (SCC) received in a histopathology laboratory and satisfied the specified criteria. Tumor was graded. Mitotic and Ki67 labeling index was calculated. Statistical analysis was performed using appropriate techniques to determine the correlation coefficient.

Results: A statistically significant difference in the Ki67 labeling index (LI) between the grades of the tumor proper was noted (P = 0.040). An increase in the mean mitotic index with histological grade was noted, which was statistically significant in the proliferating margin (P = 0.037). A statistically significant positive correlation was noted between Ki67 LI and mitotic index in tumor proper (r = 0.263, P = 0.033) and proliferative margin (r = 0.364, P = 0.003).

Conclusion: Mitotic index, a rapid and affordable tool for assessing cellular proliferation, becomes more relevant and practically effective when used in conjunction with Ki-67. Combined analysis of the two indices can be useful in the early diagnosis of possibly malignant lesions and in predicting tumor behavior.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor
  • Carcinoma, Squamous Cell* / diagnosis
  • Carcinoma, Squamous Cell* / pathology
  • Cross-Sectional Studies
  • Female
  • Head and Neck Neoplasms* / diagnosis
  • Head and Neck Neoplasms* / pathology
  • Humans
  • Immunohistochemistry / methods
  • Ki-67 Antigen* / analysis
  • Male
  • Middle Aged
  • Mitotic Index*
  • Squamous Cell Carcinoma of Head and Neck* / diagnosis
  • Squamous Cell Carcinoma of Head and Neck* / pathology

Substances

  • Ki-67 Antigen
  • Biomarkers, Tumor