The GIP receptor activates futile calcium cycling in white adipose tissue to increase energy expenditure and drive weight loss in mice

Cell Metab. 2025 Jan 7;37(1):187-204.e7. doi: 10.1016/j.cmet.2024.11.003. Epub 2024 Dec 5.

Abstract

Obesity is a chronic disease that contributes to the development of insulin resistance, type 2 diabetes (T2D), and cardiovascular risk. Glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) and glucagon-like peptide-1 (GLP-1) receptor (GLP-1R) co-agonism provide an improved therapeutic profile in individuals with T2D and obesity when compared with selective GLP-1R agonism. Although the metabolic benefits of GLP-1R agonism are established, whether GIPR activation impacts weight loss through peripheral mechanisms is yet to be fully defined. Here, we generated a mouse model of GIPR induction exclusively in the adipocyte. We show that GIPR induction in the fat cell protects mice from diet-induced obesity and triggers profound weight loss (∼35%) in an obese setting. Adipose GIPR further increases lipid oxidation, thermogenesis, and energy expenditure. Mechanistically, we demonstrate that GIPR induction activates SERCA-mediated futile calcium cycling in the adipocyte. GIPR activation further triggers a metabolic memory effect, which maintains weight loss after the transgene has been switched off, highlighting a unique aspect in adipocyte biology. Collectively, we present a mechanism of peripheral GIPR action in adipose tissue, which exerts beneficial metabolic effects on body weight and energy balance.

Keywords: GIP receptor; SERCA pathway; adipose tissue; energy expenditure; obesity; weight loss.

MeSH terms

  • Adipocytes / metabolism
  • Adipose Tissue, White* / metabolism
  • Animals
  • Calcium* / metabolism
  • Energy Metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity* / metabolism
  • Receptors, Gastrointestinal Hormone* / metabolism
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism
  • Thermogenesis
  • Weight Loss*

Substances

  • gastric inhibitory polypeptide receptor
  • Receptors, Gastrointestinal Hormone
  • Calcium
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases