Effective Nose-to-Brain Delivery of Blood-Brain Barrier Impermeant Anti-IL-1β Antibody via the Minimally Invasive Nasal Depot (MIND) Technique

ACS Appl Mater Interfaces. 2024 Dec 18;16(50):69103-69113. doi: 10.1021/acsami.4c18679. Epub 2024 Dec 10.

Abstract

Treatment of neuroinflammation and neurodegenerative diseases using biologic therapies is limited due to the blood-brain barrier (BBB). This study explores a clinically validated approach to bypass the BBB for the purposes of direct central nervous system (CNS) delivery of antibodies using the Minimally Invasive Nasal Depot (MIND) technique. Using a lipopolysaccharide (LPS)-induced mouse model of neuroinflammation, we evaluated the efficacy of MIND in delivering a BBB impermeant full-length anti-IL-1β antibody. The results demonstrated that MIND delivery resulted in a significant reduction in IL-1β levels and microglial activation in relevant brain regions, notably outperforming conventional intravenous (IV) administration. These results underscore the ability of the MIND approach to transform the treatment landscape for a range of neurodegenerative diseases by enabling the targeted delivery of otherwise BBB impermeant therapeutics.

Keywords: CNS delivery; antibody treatment; neurodegenerative diseases; neuroinflammation; nose-to-brain delivery.

MeSH terms

  • Administration, Intranasal*
  • Animals
  • Antibodies / chemistry
  • Blood-Brain Barrier* / drug effects
  • Blood-Brain Barrier* / metabolism
  • Brain / metabolism
  • Drug Delivery Systems
  • Interleukin-1beta* / metabolism
  • Lipopolysaccharides / pharmacology
  • Mice
  • Neuroinflammatory Diseases / drug therapy

Substances

  • Interleukin-1beta
  • Lipopolysaccharides
  • Antibodies