A ratiometric two-photon fluorescent probe for the quantification of nitroreductase in hypoxic neurons

Zhiquan Tang; Hong Huang; Yuanyuan Yao; Shumei Gao; Bingyong Lin; Qianshou Zong; Wanpeng Hu; Jianguo Xu; Yangang Wang; Longhua Guo

doi:10.1039/d4cc06218c

A ratiometric two-photon fluorescent probe for the quantification of nitroreductase in hypoxic neurons

Chem Commun (Camb). 2025 Jan 2;61(3):560-563. doi: 10.1039/d4cc06218c.

Authors

Affiliation

¹ College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing 314001, China. [email protected].

PMID: 39655867
DOI: 10.1039/d4cc06218c

Abstract

We developed a novel ratiometric two-photon fluorescent probe for the selective and precise detection of nitroreductase (NTR) in neurons. Using this probe, we found that hypoxic stimulation could up-regulate endogenous NTR levels.

MeSH terms

Animals
Cell Hypoxia
Fluorescent Dyes* / chemical synthesis
Fluorescent Dyes* / chemistry
Humans
Mice
Neurons* / metabolism
Nitroreductases* / analysis
Nitroreductases* / metabolism
Photons*

Substances

Fluorescent Dyes
Nitroreductases