The nucleoporin Nup153 is the anchor for Kif1a during basal nuclear migration in brain progenitor cells

Cell Rep. 2024 Dec 24;43(12):115008. doi: 10.1016/j.celrep.2024.115008. Epub 2024 Dec 11.

Abstract

Radial glial progenitors (RGPs) are highly elongated epithelial cells that give rise to most stem cells, neurons, and glia in the vertebrate cerebral cortex. During development, the RGP nuclei exhibit a striking pattern of cell-cycle-dependent oscillatory movements known as interkinetic nuclear migration (INM), which we previously found to be mediated during G1 by the kinesin Kif1a and during G2 by cytoplasmic dynein, recruited to the nuclear envelope by the nucleoporins RanBP2 and Nup133. We now identify Nup153 as a nucleoporin anchor for Kif1a, responsible for G1-specific basal nuclear migration, providing a complete model for the mechanisms underlying this basic but mysterious behavior, with broad implications for understanding brain development.

Keywords: CP: Cell biology; CP: Neuroscience; Kif1a; Nup153; brain development; cell cycle regulation; interkinetic nuclear migration; neuronal migration.

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism
  • Cell Nucleus* / metabolism
  • Humans
  • Kinesins* / metabolism
  • Mice
  • Nuclear Pore Complex Proteins* / genetics
  • Nuclear Pore Complex Proteins* / metabolism
  • Stem Cells / cytology
  • Stem Cells / metabolism

Substances

  • Nuclear Pore Complex Proteins
  • Kinesins