Oral administration of Limosilactobacillus ingluviei C37 inhibits Campylobacter jejuni colonization in chicks

Aito Murakami; Ayako Watanabe-Yanai; Taketoshi Iwata; Fu Namai; Takashi Sato; Tadashi Fujii; Takumi Tochio; Sutisa Khempaka; Takeshi Shimosato

doi:10.3389/fmicb.2024.1491039

Oral administration of Limosilactobacillus ingluviei C37 inhibits Campylobacter jejuni colonization in chicks

Front Microbiol. 2024 Nov 28:15:1491039. doi: 10.3389/fmicb.2024.1491039. eCollection 2024.

Authors

Aito Murakami^#¹, Ayako Watanabe-Yanai^#², Taketoshi Iwata², Fu Namai^{3

4}, Takashi Sato⁵, Tadashi Fujii^{6

7

8}, Takumi Tochio^{6

7

8}, Sutisa Khempaka⁹, Takeshi Shimosato^{5

10}

Affiliations

¹ Graduate School of Medicine, Science and Technology, Shinshu University, Nagano, Japan.
² Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan.
³ Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
⁴ Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
⁵ Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Kamiina, Japan.
⁶ Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Japan.
⁷ Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Toyoake, Japan.
⁸ BIOSIS Lab. Co., Ltd., Toyoake, Japan.
⁹ School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
¹⁰ Institute for Aqua Regeneration, Shinshu University, Kamiina, Japan.

^# Contributed equally.

Abstract

As the global population continues to grow, so too does the demand for poultry meat. However, the concurrent increase in the prevalence of drug-resistant bacteria has stimulated interest in the search for alternatives to antibiotics in poultry and livestock agriculture. One potential strategy is the use of probiotics. In this study, we showed that prophylactic oral administration of Limosilactobacillus ingluviei C37 (LIC37) reduced Campylobacter jejuni colonization of the cecum in cage-raised chicks, without causing significant changes in the overall diversity of gut bacteria. Further, the abundance of Blautia, another genus of probiotic bacteria, increased in the gastrointestinal tract following ingestion of LIC37 by chicks. These findings suggest that LIC37 could potentially be used as a novel probiotic agent against C. jejuni in livestock production.

Keywords: Campylobacter jejuni; Limosilactobacillus ingluviei C37; chicken; gut microbiota; probiotics.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Japan Science and Technology Agency (JST) Support for Pioneering Research Initiated by the Next Generation (SPRING) program (Grant Number JPMJSP2144 to AM), and by the Education and Research Support Fund from Shinshu University (2019–2024, to TSH).