Effect of organic selenium on performance and gill transcriptome of whiteleg shrimp Penaeus vannamei

Background: Shrimp gill health is an indicator of their overall health and environmental stress, as gills are in direct contact with the external environment. Organic selenium (OS), known for its high bioavailability and antioxidant properties, potentially enhances shrimp growth, survival, and immune responses. This study investigated the physiological features through comprehensive gill gene expression profiling and growth parameters by incorporating OS into Penaeus vannamei diets.

Methods and results: Two OS-containing products, AlkoSel (AS) and SelPlex (SL), were supplied to the diet of P. vannamei (0.90 ± 0.05 g body weight, 4.54 ± 0.5 cm length) at a dose of 0.4 mg Se kg^{- 1} ration in the final feed. Three groups were organized: an AS-fed group, an SL-fed group, and a control (C) group without additives. After a one-month feeding trial, performance indices (PI) were assessed and RNA-Seq analysis was conducted. The PI revealed that both OS products had similar growth-stimulating effects, with no significant differences between the two groups. However, both groups showed significant improvement compared to the C-group. The P. vannamei gill transcriptome was strongly impacted by the two OS products, with several KEGG pathways implicated in their metabolic mechanisms. Pathways related to energy metabolism, carbohydrate metabolism, lipid metabolism, non-specific immunity, and antioxidative responses were significant in regulating the addition of AS and SL to P. vannamei diets.

Conclusions: While SL had some higher additive effects on the gene expression profile than AS, OS from either product is an essential micronutrient that promotes growth, normal metabolic activities, and good gill health in P. vannamei during the early feeding period.