Imaging of prostate micro-architecture using three-dimensional wide-field optical coherence tomography

Marta K Skrok; Szymon Tamborski; Matt S Hepburn; Qi Fang; Mateusz Maniewski; Marek Zdrenka; Maciej Szkulmowski; Adam Kowalewski; Łukasz Szylberg; Brendan F Kennedy

doi:10.1364/BOE.537783

Imaging of prostate micro-architecture using three-dimensional wide-field optical coherence tomography

Biomed Opt Express. 2024 Nov 14;15(12):6816-6833. doi: 10.1364/BOE.537783. eCollection 2024 Dec 1.

Authors

Marta K Skrok¹, Szymon Tamborski¹, Matt S Hepburn^{1

2

3}, Qi Fang^{2

3}, Mateusz Maniewski^{4

5}, Marek Zdrenka⁵, Maciej Szkulmowski¹, Adam Kowalewski^{5

6}, Łukasz Szylberg^{4

5}, Brendan F Kennedy^{1

2

3}

Affiliations

¹ Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, 5 Grudziądzka St., 87-100 Toruń, Poland.
² Department of Electrical, Electronic and Computer Engineering, School of Engineering, The University of Western Australia, 35 Stirling Highway, Perth 6009, Western Australia, Australia.
³ BRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre Nedlands and Centre for Medical Research, The University of Western Australia, Perth, Western Australia 6009, Australia.
⁴ Department of Obstetrics, Gynaecology and Oncology, Chair of Pathomorphology and Clinical Placentology, Collegium Medicum Jan Biziel University Hospital, 75 Ujejskiego St., Bydgoszcz 85-168, Poland.
⁵ Department of Tumor Pathology and Pathomorphology, Oncology Centre, Prof Franciszek Łukaszczyk Memorial Hospital, 2 Romanowskiej St., Bydgoszcz 85-796, Poland.
⁶ Center of Medical Sciences, University of Science and Technology, 7 Kaliskiego St., Bydgoszcz 85-796, Poland.

Abstract

Prostate cancer is a global health issue that requires new diagnostic methods to provide accurate and precise visualization of prostate tissue on the micro-scale. Such methods have the potential to improve nerve-sparing surgery and to provide image guidance during prostate biopsy. In this feasibility study, we assess the potential of en face three-dimensional wide-field optical coherence tomography (OCT), covering a volumetric imaging field-of-view up to 46 × 46 × 1 mm³, to visualize micro-architecture in 18 freshly excised human prostate specimens. In each case, validation of contrast in OCT images is provided by co-registered wide-field histology images. Using this co-registration, we demonstrate that OCT can distinguish between healthy and cancerous glands at different stages, as well as visualize micro-architecture in the prostate, such as epineurium and perineurium in nerves and the tunica intima and tunica media in blood vessels.

Associated data

figshare/10.6084/m9.figshare.27169896