Real-Time and Ultrasensitive Prostate-Specific Antigen Sensing Using Love-Mode Surface Acoustic Wave Immunosensor Based on MoS2@Cu2O-Au Nanocomposites

Sensors (Basel). 2024 Nov 29;24(23):7636. doi: 10.3390/s24237636.

Abstract

Prostate-specific antigen (PSA) is a well-established tumour marker for prostatic carcinoma. In this study, we present a novel, real-time, and ultrasensitive Love-mode surface acoustic wave (L-SAW) immunosensor for PSA detection enhanced by MoS2@Cu2O-Au nanocomposite conjugation. The MoS2@Cu2O-Au nanocomposites were analyzed by SEM, XRD, and EDS. The experiments show a significant improvement in sensitivity and detection limit compared with the previous detection methods utilizing nanogold alone to detect PSA biomolecules. The experimental results show a good linear relationship when the range of PSA concentrations between 200 pg/mL and 5 ng/mL was tested. The experimental results also show good specificity against alpha 1 fetoprotein and L-tryptophan disruptors.

Keywords: Love-mode surface acoustic wave (L-SAW); immunosensor; nanocomposite; prostate-specific antigen (PSA); real time; ultrasensitive.

MeSH terms

  • Biosensing Techniques* / methods
  • Copper* / chemistry
  • Disulfides* / chemistry
  • Gold* / chemistry
  • Humans
  • Immunoassay / methods
  • Limit of Detection
  • Male
  • Molybdenum* / chemistry
  • Nanocomposites* / chemistry
  • Prostate-Specific Antigen* / blood
  • Prostatic Neoplasms / blood
  • Prostatic Neoplasms / diagnosis
  • Sound

Substances

  • Prostate-Specific Antigen
  • Gold
  • Disulfides
  • Molybdenum
  • Copper
  • molybdenum disulfide
  • cupric oxide