In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs

Denisa Jamecna; Doris Höglinger

doi:10.1007/978-1-0716-4318-1_17

In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs

Methods Mol Biol. 2025:2888:249-258. doi: 10.1007/978-1-0716-4318-1_17.

Authors

Denisa Jamecna¹, Doris Höglinger²

Affiliations

¹ Heidelberg University Biochemistry Center, Heidelberg, Germany. [email protected].
² Heidelberg University Biochemistry Center, Heidelberg, Germany. [email protected].

PMID: 39699736
DOI: 10.1007/978-1-0716-4318-1_17

Abstract

Functionalized lipid analogs are a versatile tool for studying lipid localization, metabolism and interactions in cells. As a common follow-up research, in vitro experiments are utilized to confirm specific interactors and characterize protein-lipid interactions. Here, we describe protocols that rely on commercially available photoactivatable and clickable lipids in reconstituted systems to capture protein-lipid interactions and detect lipid transport between purified proteins.

Keywords: Bifunctional lipids; Click chemistry; Gel electrophoresis; Liposomes; Photocrosslinking.

MeSH terms

Biological Transport
Click Chemistry / methods
Humans
Lipid Metabolism*
Lipids* / chemistry
Protein Binding
Proteins / chemistry
Proteins / metabolism

Substances

Lipids
Proteins