Effects on maternal and pregnancy outcomes of first-trimester malaria infection among nulliparous women from Kenya, Zambia, and the Democratic Republic of the Congo

PLoS One. 2024 Dec 20;19(12):e0310339. doi: 10.1371/journal.pone.0310339. eCollection 2024.

Abstract

Background: Few studies have assessed the impact of first-trimester malaria infection during pregnancy. We estimated this impact on adverse maternal and pregnancy outcomes.

Methods: In a convenience sample of women from the ASPIRIN (Aspirin Supplementation for Pregnancy Indicated risk Reduction In Nulliparas) trial in Kenya, Zambia, and the Democratic Republic of the Congo, we tested for first-trimester Plasmodium falciparum infection using quantitative polymerase chain reaction. We estimated site-specific effects on pregnancy outcomes using parametric g-computation.

Results: Compared to uninfected women, we observed the adjusted site-specific prevalence differences (PDs) among women with first-trimester malaria of the following pregnancy outcomes: preterm birth among Congolese (aPD = 0.06 [99% CI: -0.04, 0.16]), Kenyan (0.03 [-0.04, 0.09]), and Zambian (0.00 [-0.10, 0.20]) women; low birth weight among Congolese (0.07 [-0.03, 0.16]), Kenyan (0.01 [-0.04, 0.06]) and Zambian (-0.04 [-0.13, 0.16]) women; spontaneous abortion among Congolese (0.00 [-0.05, 0.04]), Kenyan (0.00 [-0.04, 0.04]), and Zambian (0.02 [-0.07, 0.24]) women, and anemia later in pregnancy among Congolese (0.04 [-0.09, 0.16]), Kenyan (0.05 [-0.06, 0.17]), and Zambian (0.07 [-0.12, 0.36]) women. The pooled PD for anemia later in pregnancy (26-30 weeks) was 0.08 [99% CI: 0.00, 0.16].

Conclusions: First-trimester malaria was associated with increased prevalence of anemia later in pregnancy. We identified areas for further investigation including effects of first-trimester malaria on preterm birth and low birth weight.

MeSH terms

  • Adult
  • Democratic Republic of the Congo / epidemiology
  • Female
  • Humans
  • Infant, Low Birth Weight
  • Infant, Newborn
  • Kenya / epidemiology
  • Malaria, Falciparum* / epidemiology
  • Malaria, Falciparum* / parasitology
  • Parity
  • Plasmodium falciparum / isolation & purification
  • Pregnancy
  • Pregnancy Complications, Parasitic* / epidemiology
  • Pregnancy Outcome*
  • Pregnancy Trimester, First*
  • Premature Birth / epidemiology
  • Prevalence
  • Young Adult
  • Zambia / epidemiology

Grants and funding

This work was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development grants: UG1 HD076465 (CLB, AT, AL, MB), UG1 HD078437 (WAC, EC, MM), UG1HD078438 (SJ, SS, RLG), UG1HD076461 (EAL, SLB, OAE, FE, PN), and UG1HD076457 (RJD, MH), and U24HD092094 (TN, JH-F, EMM). Dr. Marion Koso-Thomas is a project officer within the National Institute of Child Health and Human Development (NICHD) Global Network and provided oversight of protocol implementation and monitoring safety and performance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.