Decorin alleviates non-alcoholic fatty liver disease in rats with polycystic ovary syndrome

Hany A Elkattawy; Amira Ebrahim Alsemeh; Lashin Saad Ali; Mona Mostafa Ahmed; Asmaa Monir Eltaweel; Farha M Shaikh; Ahmed Behiry; Ahmed El-Sayed Hassan; Deema Kamal Sabir; Dalia Mahmoud Abdelmonem Elsherbini; Sahar K Ali; Madaniah Omar Zakari; Moaz Abdullah Mojaddidi; Ehab Kamal Ali; Yasser M Elbastawisy; Shimaa Hadhoud

doi:10.1016/j.tice.2024.102689

Decorin alleviates non-alcoholic fatty liver disease in rats with polycystic ovary syndrome

Tissue Cell. 2024 Dec 15:93:102689. doi: 10.1016/j.tice.2024.102689. Online ahead of print.

Authors

Hany A Elkattawy¹, Amira Ebrahim Alsemeh², Lashin Saad Ali³, Mona Mostafa Ahmed⁴, Asmaa Monir Eltaweel⁵, Farha M Shaikh⁶, Ahmed Behiry⁷, Ahmed El-Sayed Hassan⁸, Deema Kamal Sabir⁹, Dalia Mahmoud Abdelmonem Elsherbini¹⁰, Sahar K Ali¹¹, Madaniah Omar Zakari¹², Moaz Abdullah Mojaddidi¹³, Ehab Kamal Ali¹⁴, Yasser M Elbastawisy¹⁵, Shimaa Hadhoud¹⁶

Affiliations

¹ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Diriyah 13713, Saudi Arabia. Electronic address: [email protected].
² Human Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt. Electronic address: [email protected].
³ Department of Basic Medical Sciences, Faculty of Dentistry, Al-Ahliyya Amman University, P.O. Box 19328, Amman, Jordan; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: [email protected].
⁴ Pathology Department, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt. Electronic address: [email protected].
⁵ Human Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt; College of Medicine, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh 11481, Saudi Arabia; King Abdullah International Medical Research Center, Riyadh 11481, Saudi Arabia. Electronic address: [email protected].
⁶ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Diriyah 13713, Saudi Arabia. Electronic address: [email protected].
⁷ Department of Tropical Medicine and Endemic Diseases, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt. Electronic address: [email protected].
⁸ Medical Physiology Department, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt; Department of Basic Medical Sciences, College of Medicine, Sulaiman Al-Rajhi University, Bukayriah 51941, Saudi Arabia. Electronic address: [email protected].
⁹ Department of Medical Surgical Nursing, College of Nursing, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: [email protected].
¹⁰ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: [email protected].
¹¹ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Diriyah 13713, Saudi Arabia; Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt. Electronic address: [email protected].
¹² Department of Basic Medical Sciences, College of Medicine, Taibah University, Medina, Saudi Arabia. Electronic address: [email protected].
¹³ Department of Basic Medical Sciences, College of Medicine, Taibah University, Medina, Saudi Arabia. Electronic address: [email protected].
¹⁴ Department of Anatomy and Embryology, Faculty of Medicine, AL-Azhar University, New Damietta, Egypt. Electronic address: [email protected].
¹⁵ Department of Basic Medical Sciences, College of Medicine, Al-Rayan Colleges, Al-Madinah, Saudi Arabia; Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: [email protected].
¹⁶ Medical Physiology Department, Faculty of Medicine, Zagazig University, P.O. Box 44519, Zagazig, Egypt. Electronic address: [email protected].

PMID: 39705869
DOI: 10.1016/j.tice.2024.102689

Abstract

Endocrine multisystem defect polycystic ovary syndrome (PCOS) causes hyperandrogenism and infertility. Half of PCOS women have (non-alcoholic fatty liver disease) NAFLD, which increases metabolic disease risk. We tested decorin's effect on NAFLD and related processes in PCOS. NAFLD+PCOS, PCOS+decorin, and control rats were studied. Decorin was evaluated on NAFLD/PCOS rats. Test group rats received HF for eight weeks to generate NAFLD. The rats got 1 mg/kg letrozole orally daily for 21 days to diagnosis PCOS. Afterward, rats got injectable decorin for 14 days. Body weight, liver weight, liver coefficient Abdominal Circumference (AC) and body mass index (BMI) were determined. Blood triglycerides (TG), total cholesterol, LDL-c, AST, and glucose were measured. The insulin, testosterone, estrogen, LH, and FSH were measured by ELISA. GPx, SOD, MDA, TNF-, and Caspase-3 liver immunohistochemistry were evaluated. NAFLD liver tissues in PCOS models showed biochemical and histological alterations. NAFLD+PCOS raised BMI, AC, liver weight, and coefficient. Blood glucose, insulin resistance, TG, ALT, and AST increased. Lipid abnormalities (TG, cholesterol, LDL-c, and HDL-c), oxidative stress markers (MDA, SOD, and GPx), and liver dysfunction were found. Low serum E2 and high T supported PCO. Decorin reduced model rat BMI, liver weight, coefficient, insulin resistance, TG, ALT, and AST. It reduced liver inflammation, improved liver extract lipids, and normalized MDA, SOD, and GPx. In the model group, decorin lowered serum T, E2, LH, caspase 3, and TNF-alpha. Decorating improved NAFLD/PCOS group liver histology and function. Decorin reduces hepatosteatosis by reducing liver inflammation, oxidative stress, and dyslipidemia.

Keywords: Decorin; Insulin; Nonalcoholic fatty liver diseases; Polycystic ovary syndrome; Rat.