Synergistic effects of ozonation pretreatment and trace phosphate on water quality health risk and microbial stability in simulated drinking water distribution systems

The proliferation and chlorine resistance of pathogenic bacteria in drinking water distribution systems (DWDSs) pose a serious threat to human health. In this study, the synergistic effects of ozonation pretreatment and trace phosphate on water quality health risk and microbial stability were investigated in the small-scale DWDSs simulated by biofilms annular reactors with cast iron coupons. The results indicated that ozonation of drinking water containing trace phosphate was equivalent to increasing microbial carbon and phosphorus sources, further leading to the rapid proliferation of opportunistic pathogens (OPs) in subsequent DWDSs. Under the influent condition of ozonation pretreatment and 0.6 mg/L phosphate, the gene copy numbers of living Legionella spp., Mycobacterium spp., and Acanthamoeba spp. reached up to 1.50 × 10⁴, 1.21 × 10⁴, and 2.29 × 10⁴ gene copies/mL, respectively. The extracellular polymeric substances from suspended biofilms in DWDSs exhibited higher content, molecular weight, and flocculating efficiency, contributing to the improvement of microbial chlorine resistance. Meanwhile, more Fe₃O₄ appeared in the corrosion products, which enhanced the extracellular electron transfer via cytochrome c and weakened the electrostatic repulsion between corrosion products and microbes in DWDSs. Finally, more active OP growth and microbial metabolic activity occurred in DWDSs. This study revealed that ozonation pretreatment and trace phosphate, as a green technology and an inconspicuous nutrient, respectively, can trigger significant microbial health risks in subsequent DWDSs. Therefore, the phosphate in drinking water should be more strictly restricted when ozonation technology is used in waterworks, especially without a biofiltration treatment process.