Antioxidant and antibacterial coconut mesocarp polyphenol hydrogel dressing based on PVA/quaternary chitosan/sodium alginate with β-glycerophosphate

This study developed PQSp wound dressing hydrogels (S0-S6) using polyvinyl alcohol (PVA), quaternary chitosan (QCS), and sodium alginate (SA) as the matrix, with the addition of coconut mesocarp polyphenol (P-CTP, 0.1 %, 0.5 %, and 1.0 %) and β-glycerophosphate disodium (GP, 1.0 %) through a freeze-thaw method. Compared to hydrogels without P-CTP and GP (S0), the tensile strength of S1-S6 increased from 0.08 MPa to 0.45 MPa, elongation at break improved from 200 % to 320 %, and the swelling ratio decreased from 186 % to 82 % due to the effects of P-CTP and GP, while maintaining water content above 80 %, ensuring a moist environment for wound healing. Their thermal stability was also improved. SEM, FTIR, and XPS results confirmed enhanced crosslinking within the multi-network of the hydrogels, attributed to the increased hydrogen bonding from GP and P-CTP, independent of chemical crosslinking. However, antioxidant and antibacterial activities were dose-dependent only on P-CTP, with S3 and S6 showing the best effects. CAM and chicken embryo assays confirmed the hydrogels' non-toxicity and biocompatibility. These findings suggest that PQSp hydrogels, with their excellent mechanical properties, bioactivity, and safety, hold great potential for advanced wound dressing applications and provide a reference for expanding the application range of P-CTP.