Kinetic studies of bifurcating flavoproteins

Russ Hille; Dimitri Niks; Wayne Vigil Jr; Jessica Tran; Steve Ortiz; Kevin Menjivar; Derek Nguyen

doi:10.1016/j.abb.2024.110278

Kinetic studies of bifurcating flavoproteins

Arch Biochem Biophys. 2024 Dec 19:764:110278. doi: 10.1016/j.abb.2024.110278. Online ahead of print.

Authors

Russ Hille¹, Dimitri Niks², Wayne Vigil Jr³, Jessica Tran⁴, Steve Ortiz³, Kevin Menjivar⁴, Derek Nguyen⁴

Affiliations

¹ Department of Biochemistry, University of California, Riverside, USA. Electronic address: [email protected].
² Department of Biochemistry, University of California, Riverside, USA.
³ Department of Biochemistry, University of California, Riverside, USA; The Biophysics Graduate Program, University of California, Riverside, USA.
⁴ Department of Biochemistry, University of California, Riverside, USA; The Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, USA.

PMID: 39709109
DOI: 10.1016/j.abb.2024.110278

Abstract

Since their original proposal in 2008, a number of broadly distributed flavoprotein systems catalyzing electron bifurcation have been identified that play key roles in the bioenergetics of anaerobic bacteria and archaea. While the overall thermodynamics of flavin-based electron bifurcation are now quite well-understood, the same cannot be said of their kinetic behavior. The present account represents a summary of results obtained with several electron-electron bifurcating systems, shamelessly focusing on work done in the authors' laboratory.

Keywords: Electron bifurcation; Electron transfer; Flavoproteins; Rapid-reaction kinetics.

Publication types

Review