Effects of PGK1 on immunoinfiltration by integrated single-cell and bulk RNA-sequencing analysis in sepsis

Front Immunol. 2024 Dec 6:15:1449975. doi: 10.3389/fimmu.2024.1449975. eCollection 2024.

Abstract

Background: Sepsis, a life-threatening organ dysfunction caused by a dysregulated immune response to infection, remains a significant global health challenge. Phosphoglycerate kinase 1 (PGK1) has been implicated in regulating inflammation and immune cell infiltration in inflammatory conditions. However, the role of PGK1 in sepsis remains largely unexplored.

Methods: Four microarray datasets and a high throughput sequencing dataset were acquired from GEO database to reveal the PGK1 expression in patients of sepsis. Quantitative real-time PCR and western blotting was then used to validate the PGK1 level. Additionally, microarray and single-cell RNA sequencing data integration, including gene set enrichment analysis (GSEA), KEGG and GO functional enrichment analysis, immune infiltration analysis, and single-cell sequencing analysis, were performed to elucidate the role of PGK1 in sepsis.

Results: Our results revealed a significant upregulation of PGK1 in sepsis patients, with the area under the ROC curve (AUC) exceeding 0.9 across multiple datasets, indicating PGK1's strong potential as a diagnostic biomarker. Notably, PGK1 was enriched in key immune-related pathways, including the TNF signaling pathways, and leukocyte transendothelial migration, suggesting its involvement in immune regulation. Furthermore, PGK1 expression showed a positive correlation with the levels of inflammatory mediators CXCL1, CXCL16, and the chemokine receptor CCR1. In terms of immune cell infiltration, PGK1 was positively correlated with naive B cells, resting memory CD4 T cell, gamma delta T cells, M0 macrophages, eosinophils and negatively correlated with plasma cells, CD8 T cells, activated memory CD4 T cell, Tregs, activated dendritic cells.

Conclusions: This study concluded that PGK1 served as a novel diagnostic biomarker for sepsis, with potential implications for prognosis and immune regulation. The significant upregulation of PGK1 in sepsis patients and its association with immune-related pathways and cell types highlight its potential role in the pathogenesis of sepsis.

Keywords: PGK1; biomarker; immune infiltration; sepsis; single-cell RNA-sequencing.

MeSH terms

  • Biomarkers*
  • Female
  • Gene Expression Profiling
  • Humans
  • Male
  • Middle Aged
  • Phosphoglycerate Kinase* / genetics
  • Sepsis* / diagnosis
  • Sepsis* / genetics
  • Sepsis* / immunology
  • Sequence Analysis, RNA
  • Single-Cell Analysis*

Substances

  • Phosphoglycerate Kinase
  • PGK1 protein, human
  • Biomarkers

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The research was supported by Guangdong Basic and Applied Basic Research Foundation (2019A1515110488), Shenzhen Municipal Science and Technology Innovation Commission (JCYJ20210324123414040 and JCYJ20230807110715030), Guangdong Pearl River Talent Program (2021QN02Y108), and Shenzhen Medical Research Fund (A2402020).