Proteomics-Based Analysis of Laser-Capture Micro-dissected, Formalin-Fixed Paraffin-Embedded Tissue Samples

Teresa Mendes Maia; Delphi Van Haver; Sara Dufour; Malaïka Van der Linden; Amélie Dendooven; Francis Impens; Simon Devos

doi:10.1007/978-1-0716-4298-6_20

Proteomics-Based Analysis of Laser-Capture Micro-dissected, Formalin-Fixed Paraffin-Embedded Tissue Samples

Methods Mol Biol. 2025:2884:333-354. doi: 10.1007/978-1-0716-4298-6_20.

Authors

Teresa Mendes Maia^{1

2

3}, Delphi Van Haver^{1

2

3}, Sara Dufour^{1

2

3}, Malaïka Van der Linden^{4

5}, Amélie Dendooven^{4

5}, Francis Impens^{1

2

3}, Simon Devos^{6

7

8}

Affiliations

¹ VIB-UGent Center for Medical Biotechnology, Ghent, Belgium.
² Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
³ VIB Proteomics Core, Ghent, Belgium.
⁴ Department of Pathology, Ghent University Hospital, Ghent, Belgium.
⁵ Department of Diagnostic Sciences, Ghent University, Ghent, Belgium.
⁶ VIB-UGent Center for Medical Biotechnology, Ghent, Belgium. [email protected].
⁷ Department of Biomolecular Medicine, Ghent University, Ghent, Belgium. [email protected].
⁸ VIB Proteomics Core, Ghent, Belgium. [email protected].

PMID: 39716012
DOI: 10.1007/978-1-0716-4298-6_20

Abstract

The ability to bring spatial resolution to omics studies enables a deeper understanding of cell populations and interactions in biological tissues. In the case of proteomics, single-cell and spatial approaches have been particularly challenging, due to limitations in sensitivity and throughput relative to other omics fields. Recent developments at the level of sample handling, chromatography, and mass spectrometry have set the stage for proteomics to be established in these new disciplines.

Keywords: Amyloidosis; DIA; FFPE; LCM; Spatial proteomics.

MeSH terms

Animals
Chromatography, Liquid / methods
Formaldehyde* / chemistry
Humans
Laser Capture Microdissection* / methods
Mass Spectrometry / methods
Paraffin Embedding* / methods
Proteome
Proteomics* / methods
Tissue Fixation* / methods

Substances

Formaldehyde
Proteome