The expression of glycolysis-related proteins in urine significantly increases after running

Tian Zhao; Tianci Liu; Tao Li; Shengcun Chen; Lupeng Wang; Man Zhang

doi:10.3389/fphys.2024.1481741

The expression of glycolysis-related proteins in urine significantly increases after running

Front Physiol. 2024 Dec 9:15:1481741. doi: 10.3389/fphys.2024.1481741. eCollection 2024.

Authors

Tian Zhao^{1

2}, Tianci Liu^{2

3}, Tao Li^{2

3}, Shengcun Chen^{2

3}, Lupeng Wang^{2

3}, Man Zhang^{2

3

4}

Affiliations

¹ College of Information Engineering, Hangzhou Dianzi University, Hangzhou, China.
² Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China.
³ Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
⁴ Institute of Regenerative Medicine and Laboratory Technology Innovation, Qingdao University, Qingdao, China.

Abstract

Objective: Glucose metabolism is the main way in which cells obtain energy during exercise and plays an important role in exercise. The purpose of this study was to explore the changes in the expression of glucose metabolism-related proteins in urine after running, and finally applied to the monitoring of running training.

Methods: Urine samples were collected before and after running, and urine proteomics information was collected to explore the expression of proteins in the urine using LC-MS/MS in DDA mode and DIA mode. Receiver operating characteristic (ROC) curve was drawn to evaluate the value of target proteins in monitoring running training.

Results: A total of 140 proteins were identified using LC-MS/MS in DDA mode, of which 49 urine proteins showed increased expression after running. KEGG analysis revealed that glucose metabolism-related proteins are mainly concentrated in glycolysis. There were six glycolysis-related proteins, among which urine proteins PKM, TPI1, ENO1 and LDHB were significantly increased after running (P < 0.05). This changes in urine proteins PKM, TPI1, ENO1 and LDHB were further verified by the results of LC-MS/MS in DIA mode. The concentrations of the urine proteins TPI1, ENO1 and LDHB showed a significant linear relationship with PKM. ROC curve analysis showed that PKM, TPI1, ENO1 and LDHB proteins in urine had good monitoring values for running training.

Conclusion: The expression of glycolysis-related proteins PKM, TPI1, ENO1 and LDHB in urine was significantly increased after running, which may be applied to the monitoring of running training.

Keywords: biomarkers; glycolysis-related proteins; running; training; urine.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. Thanks are due to all the volunteers for their generous donation of urine samples. This work was supported by Talent Training Plan during the “14th Five-Year Plan” period of Beijing Shijitan Hospital Affiliated to Capital Medical University (2023LJRCZM).