Crystallization and preliminary X-ray crystallographic studies of AfeH from Acimetobacter sp. DL-2

Acta Crystallogr F Struct Biol Commun. 2025 Jan 1. doi: 10.1107/S2053230X24012275. Online ahead of print.

Abstract

Fenoxaprop-P-ethyl (FE) is widely applied as a post-emergence aryloxyphenoxy propionate (AOPP) herbicide in agriculture. A novel FE hydrolase esterase from Acinetobacter sp. DL-2 (AfeH) was identified which belongs to the family IV carboxylesterases and shows less than 30% identity to other reported homologues with known structure. In order to understand the catalytic mechanism, recombinant AfeH was prepared in Escherichia coli and crystallized using the sitting-drop vapor-diffusion method. X-ray diffraction data were collected to 1.9 Å resolution. The crystal belonged to space group P1211, with unit-cell parameters a = 84.27, b = 46.74, c = 258.68 Å. The Matthews coefficient (VM) was calculated to be 2.43 Å3 Da-1, which corresponds to a solvent content of 49.4%, suggesting the presence of three monomers in the crystallographic asymmetric unit. The crystal was assessed to be suitable for further structural determination, which is currently in progress.

Keywords: Acimetobacter sp. DL-2; AfeH; X-ray crystallography; carboxylesterases; family IV.