Isolation and characterization of syncytiotrophoblast plasma membrane from human placenta

FEBS Lett. 1985 Feb 11;181(1):33-8. doi: 10.1016/0014-5793(85)81108-x.

Abstract

Human full-term syncytiotrophoblast plasma membranes isolated by mechanical procedures (sieving and ultrasonic disintegration), purified by phase centrifugation, form a single band of 1.052 +/- 0.002 g/ml density in percoll gradient. The purity of the preparation was assessed by electron microscopy, enzyme analysis and beta 2-microglobulin determination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fractionation
  • Cell Membrane / ultrastructure
  • Centrifugation, Density Gradient
  • Female
  • Humans
  • Microscopy, Electron
  • Placenta / ultrastructure*
  • Pregnancy
  • Trophoblasts / ultrastructure*