Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Jinsong Yang; Songsong Zhao; Wenbo Zhi; Tianyu Lu; Huahua Qiao; Wei Liu; Ying Dou; Haisheng Tan; Hanlin Zhou

doi:10.3389/fmicb.2024.1468577

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Front Microbiol. 2024 Dec 11:15:1468577. doi: 10.3389/fmicb.2024.1468577. eCollection 2024.

Authors

Jinsong Yang^#¹, Songsong Zhao^#¹, Wenbo Zhi^#¹, Tianyu Lu¹, Huahua Qiao¹, Wei Liu¹, Ying Dou¹, Haisheng Tan², Hanlin Zhou^{3

4}

Affiliations

¹ College of Food Science and Engineering, Hainan University, Haikou, Hainan, China.
² College of Materials Science and Engineering, Hainan University, Haikou, Hainan, China.
³ Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.
⁴ Key Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation and Utilization of Cassava, Key Laboratory of Ministry of Agriculture and Rural Affairs for Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.

^# Contributed equally.

Abstract

The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

Keywords: Lactobacillus; high-throughput sequencing; microbial communities; protoplast fusion; silage.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (No. 31960678), the Hainan Province Science and Technology Special Fund (ZDYF2022XDNY186), funded by the Key Research and Development Projects of Hainan province (GHYF2022004).