Enhancing astaxanthin accumulation in immobilized Haematococcus pluvialis via alginate hydrogel membrane

Yating Zhang; Xixi Wang; Dong Su; Ling Zhao; Kailiang Leng; Junkui Miao; Yueqin Yu

doi:10.1016/j.ijbiomac.2024.139145

Enhancing astaxanthin accumulation in immobilized Haematococcus pluvialis via alginate hydrogel membrane

Int J Biol Macromol. 2024 Dec 24:139145. doi: 10.1016/j.ijbiomac.2024.139145. Online ahead of print.

Authors

Yating Zhang¹, Xixi Wang², Dong Su³, Ling Zhao¹, Kailiang Leng⁴, Junkui Miao⁵, Yueqin Yu⁶

Affiliations

¹ State Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; Qingdao Engineering Research Center of Polar Fishery Resources Exploitation, Key Laboratory of Sustainable Marine Fishery Development of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
² Qingdao Engineering Research Center of Polar Fishery Resources Exploitation, Key Laboratory of Sustainable Marine Fishery Development of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
³ State Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁴ Qingdao Engineering Research Center of Polar Fishery Resources Exploitation, Key Laboratory of Sustainable Marine Fishery Development of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laoshan Laboratory, Qingdao 266237, China. Electronic address: [email protected].
⁵ Qingdao Engineering Research Center of Polar Fishery Resources Exploitation, Key Laboratory of Sustainable Marine Fishery Development of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. Electronic address: [email protected].
⁶ State Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: [email protected].

PMID: 39725098
DOI: 10.1016/j.ijbiomac.2024.139145

Abstract

Immobilized cultivation is anticipated to be effective for enhancing both biomass and astaxanthin accumulation in Haematococcus pluvialis (H. pluvialis). A novel fabrication method of alginate hydrogel membrane (AHM) was introduced for immobilized cultivation of H. pluvialis. This method incorporates cotton gauze into a hydrogel with a low sodium alginate (SA) concentration of 0.5 %, utilizing endogenous calcification. The optimized culture strategy achieved a peak astaxanthin productivity of 256.3 mg·m^-2·d^-1 with an inoculum of 16 g·m^-2 under light irradiation of 300 μmol·m^-2·s^-1 on day 4, resulting in a 70.8 % increase in astaxanthin yield over the control group. Furthermore, a recovery method for H. pluvialis and SA from AHM was explored, using Na₂CO₃ to disintegrate AHM to recover all the microalgal cells and SA with a recovery rate of 88.7 %. Collectively, these findings suggest that immobilized cultivation using AHM is an effective strategy for boosting biomass and astaxanthin accumulation in H. pluvialis.

Keywords: Haematococcus pluvialis; Hydrogel membrane; Immobilized cultivation.