Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor

Zhen Tian; Hongzhuan Xuan; Yicun Yao; Shengyu Hao; Zhichao Zhang; Bingyuan Zhang; Jingao Zhang; Liqiang Zhang; Xinzhu Sang; Jinhui Yuan; Gerald Farrell; Qiang Wu

doi:10.1038/s44172-024-00332-y

Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor

Commun Eng. 2024 Dec 26;3(1):185. doi: 10.1038/s44172-024-00332-y.

Authors

Zhen Tian^#^{1

2}, Hongzhuan Xuan^#³, Yicun Yao², Shengyu Hao², Zhichao Zhang¹, Bingyuan Zhang², Jingao Zhang¹, Liqiang Zhang², Xinzhu Sang¹, Jinhui Yuan⁴, Gerald Farrell⁵, Qiang Wu^{6

7

8}

Affiliations

¹ State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China.
² School of Physics Sciences and Information Technology, Liaocheng University, Liaocheng, China.
³ School of Life Science, Liaocheng University, Liaocheng, China.
⁴ State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China. [email protected].
⁵ Photonics Research Centre, School of Electrical and Electronic Engineering, City Campus, Technological University Dublin, Dublin, Ireland.
⁶ State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China. [email protected].
⁷ Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne, United Kingdom. [email protected].
⁸ Key Laboratory of Optoelectronic Information Science and Technology of Jiangxi Province, Nanchang Hangkong University, Nanchang, China. [email protected].

^# Contributed equally.

Abstract

Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin's anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.

Abstract

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