Zinc-lysine and iron-lysine mitigate chromium toxicity in pearl millet (Pennisetum glaucum) through modulating photosynthetic and antioxidant system and inhibiting chromium uptake and translocation

Chromium (Cr) is an ever-present abiotic stress that negatively affects crop cultivation and production worldwide. High rhizospheric Cr concentrations inhibit nutrients uptake and their translocation to aboveground parts, thus can affect the growth and development of crop plants. This experiment was designed to evaluate the effects of sole and combined zinc-lysine and iron-lysine applications on photosynthetic efficacy, antioxidative defense, oxidative stress, and nutrient uptake and translocation under Cr stress. Chromium stress exhibited toxic effects on the growth, physiological, and biochemical indices of pearl millet. The combined application of zinc-lysine and iron-lysine significantly decreased malondialdehyde (MDA; 25%) and hydrogen peroxide (H₂O₂; 22.44%), while increased superoxide dismutase (SOD; 19.75%), catalase (CAT; 26.16%), peroxidase (POD; 19.62%), and ascorbate peroxidase (APX; 23.52%) activities under Cr toxicity compared to the control treatment. In addition, the combined application of zinc-lysine and iron-lysine effectively improved net photosynthesis (43.63%), stomatal conductance (20.05%), transpiration rate (20.14%), internal CO₂ concentration (34.28%), total chlorophyll (43.12%), relative water content (23.95%), membrane stability index (32.77%), and proline content (25.53%) under stress condition and compared with control. Our results also indicated that the combined application of zinc-lysine and iron-lysine decreased Cr uptake in both shoot and root by 31.25% and 32%, and increased zinc and iron uptake by 39.28% and 36.67%, respectively, over the control, under Cr stress conditions. Moreover, under stress conditions, combined zinc-lysine and iron-lysine effectively improved growth traits particularly shoot and root dry weights, by 8% and 36.84%, respectively, over the control treatment. Overall, our results demonstrated that combined zinc-lysine and iron-lysine was more effective in mitigating Cr toxicity in pearl millet compared with the sole application of these treatments or the control.