Important ecophysiological roles of Nocardiopsis in lignocellulose degradation during aerobic compost with humic acid addition

Improving lignocellulose degradation and organic matter conversion in agricultural and livestock wastes remains a great challenge. Here, the contribution of humic acid (HA) to lignocellulose degradation was investigated, focusing on the abundance of key microbial species and carbohydrate-active enzymes during aerobic composting. The results demonstrated that the addition of HA not only increased the complexity of the microbial network, but also enhanced the positive interaction between microorganism. The abundance of phylum Actinobacteria related to lignin degradation was significantly increased, especially genus Nocardiopsis (50.97 %), and Nocardiopsis was significantly positively correlated with HA and humus (HS) (p < 0.05). Additionally, the abundance of GH (43.45%) and AA (5.88%) enzymes and the activation of metabolic pathways of AA, carbohydrates and energy were significantly increased (p < 0.05). Remarkably, the quantity of lignocellulose-degrading genes and carbohydrate-active enzymes experienced a marked boost (p < 0.05), with the peak abundance observed in Nocardiopsis. The structural equation model revealed that the addition of HA boosted the abundance of Nocardiopsis, which in turn amplified lignocellulose degradation by up-regulating lignocellulose degradation genes and enhancing carbohydrase activity, and facilitating the conversion of HA and FA. The lignocellulose degradation experiment verified that Nocardiopsis alba exhibited good ability in the degradation of cellulose and hemicellulose. These findings provided a novel perspective on the mechanisms underlying lignocellulose degradation, and broaden the understanding of the ecophysiological role of Nocardiopsis in composting system.