Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.
Keywords: DpDCA gene; Dunaliella parva; Enzymatic activity; Gene expression; Organic content.
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.