The Cardioprotective Effect of Ginseng Derived Exosomes via Inhibition of Oxidative Stress and Apoptosis

Ginsenosides possess potential protective effects against cisplatin (CDDP)-induced toxicity, but the limited bioavailability of ginsenosides hampered their therapeutic application. Ginseng exosomes (G-Exo), which are active ingredients in ginseng, exhibit excellent biocompatibility and low immunogenicity. Here, G-Exo were isolated from ginseng roots through a combination of ultracentrifugation and sucrose gradient centrifugation techniques. Subsequently, the potential protective effect of G-Exo on CDDP induced cardiotoxicity, and its underlying mechanisms were explored. The findings demonstrated that G-Exo effectively mitigated CDDP-induced oxidative stress and apoptosis in vitro. Moreover, in vivo experiments revealed that G-Exo significantly inhibited the increases in serum cardiac troponin T (cTnT), creatine kinase (CK), and lactate dehydrogenase (LDH) levels in mice induced by CDDP. Histological assessment and tissue staining further corroborated that G-Exo alleviated the cardiac tissue damage and apoptosis caused by CDDP. Mechanistically, G-Exo were found to alleviate CDDP-induced apoptosis through blocking the MAPK signaling. Collectively, these results suggest that G-Exo hold the potential to mitigate cisplatin-induced cardiac injury by regulating the MAPK pathway, thereby highlighting the therapeutic potential of G-Exo as a protective agent against CDDP-induced cardiotoxicity.