Contribution of the microbial community to operational stability in an anammox reactor: Neutral theory and functional redundancy perspectives

A comprehensive understanding of microbial assembly is essential for achieving stable performance in biological wastewater treatment. Nevertheless, few studies have quantified these phenomena in detail, particularly in anammox-based processes. This study integrated mathematical and microbial approaches to analyze a 330-day anammox reactor with stable nitrogen removal efficiency (97 - 99%) despite changes in the high nitrogen loading rate, nitrogen concentration, and hydraulic retention time. A high value of functional redundancy (0.82) was obtained, with 84.6% of the microbial species following the neutral community model in stochastic processes, thus maintaining the stability of the dominant species and function in the microbial community. This study represents an initial attempt to quantify and evaluate the importance of functional redundancy in an anammox reactor. Based on these findings, engineering strategies have also been proposed to preserve high functional redundancy in stabilizing system performance under varying operating conditions.