Diagnostic value of SAT-TB in stool and urine samples for intestinal and urinary tuberculosis

Zhao Wei; Muhammad Tahir Khan; Feng Zhiyu; Wang Min; Zhang Hong; Yuan Yuan; Wu Di; Chen Zeying; Huang Xianlin; Tan Yaoju; Kuang Haobin

doi:10.1016/j.diagmicrobio.2024.116672

Diagnostic value of SAT-TB in stool and urine samples for intestinal and urinary tuberculosis

Diagn Microbiol Infect Dis. 2024 Dec 26;111(3):116672. doi: 10.1016/j.diagmicrobio.2024.116672. Online ahead of print.

Authors

Zhao Wei¹, Muhammad Tahir Khan², Feng Zhiyu³, Wang Min⁴, Zhang Hong⁵, Yuan Yuan⁶, Wu Di⁷, Chen Zeying⁸, Huang Xianlin⁹, Tan Yaoju¹⁰, Kuang Haobin¹¹

Affiliations

¹ Graduate School, Guangzhou Medical University, Guangzhou 511436, PR China. Electronic address: [email protected].
² State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China; Institute of Molecular Biology and Biotechnology, The University of Lahore, KM Defence Road, Lahore 58810, Pakistan. Electronic address: [email protected].
³ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁴ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁵ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁶ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁷ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁸ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
⁹ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
¹⁰ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].
¹¹ State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangdong 510095, PR China. Electronic address: [email protected].

PMID: 39742705
DOI: 10.1016/j.diagmicrobio.2024.116672

Abstract

Background: The simultaneous amplification/testing for tuberculosis (SAT-TB) targets specific 16s rRNA for detecting Mycobacterium tuberculosis in real-time.

Objective: To evaluate SAT-TB's performance in detecting intestinal and urinary TB using stool and urine samples.

Methods: Stool (94) and urine samples (69) (From 2021 to 2022), were collected from pulmonary combined with suspected intestinal or urinary tuberculosis. Simultaneous detection of Mycobacterium tuberculosis was performed using the SAT-TB method, Xpert MTB/RIF assay, and MGIT960 culture.

Results: For stool samples, the sensitivity, specificity, and area under the curve (AUC) were 53.33 %, 93.88 %, and 0.736 for SAT-TB; 60 %, 81.63 %, and 0.708 for Xpert; and 40 %, 95.92 %, and 0.680 for MGIT960. For urine samples, the sensitivity, specificity, and AUC for SAT-TB, Xpert, and MGIT960 were 27.27 %, 98.28 %, 0.628; 54.55 %, 96.35 %, 0.755; and 45.45 %, 100 %, 0.727, respectively.

Conclusion: ROC analysis indicated that SAT-TB had the highest diagnostic efficacy for stool samples when tested individually.

Keywords: Intestinal tuberculosis; SAT; SAT-TB; Urinary tuberculosis.