Construction of the KNOX-BELL interaction network and functional analysis of CmBLH2 under cold stress in Chrysanthemum morifolium

Peixue Liu; Jing Tang; Yating Lei; Lingling Zhang; Jingxuan Ye; Chun Wang; Lijie Zhou; Ye Liu; Zhenxing Wang; Jiafu Jiang; Fadi Chen; Aiping Song

doi:10.1016/j.ijbiomac.2024.139365

Construction of the KNOX-BELL interaction network and functional analysis of CmBLH2 under cold stress in Chrysanthemum morifolium

Int J Biol Macromol. 2024 Dec 30:139365. doi: 10.1016/j.ijbiomac.2024.139365. Online ahead of print.

Authors

Peixue Liu¹, Jing Tang², Yating Lei³, Lingling Zhang⁴, Jingxuan Ye⁵, Chun Wang⁶, Lijie Zhou⁷, Ye Liu⁸, Zhenxing Wang⁹, Jiafu Jiang¹⁰, Fadi Chen¹¹, Aiping Song¹²

Affiliations

¹ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
² State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
³ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁴ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁵ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁶ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁷ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁸ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
⁹ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
¹⁰ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
¹¹ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].
¹² State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing 210014, China. Electronic address: [email protected].

PMID: 39743079
DOI: 10.1016/j.ijbiomac.2024.139365

Abstract

The three-amino-acid-loop-extension (TALE) homeodomain transcription factor family, including the KNOX and BELL subfamilies, is one of the largest gene families in plants. This family encodes plant-specific transcription factors that play critical roles in regulating plant growth, development, and stress responses. However, their interaction network, as well as resistant functional mechanism in is rarely reported. In this study, 60 members of the TALE transcription factor family in chrysanthemum (Chrysanthemum morifolium) were systematically identified. These genes are distributed across 27 chromosomes, with most originating from whole-genome duplication events. Through comprehensive analyses of evolution, gene structure, and cis-regulatory elements, the expression patterns of these genes were elucidated, highlighting their roles in various developmental stages and stress responses, thereby expanding our understanding of the TALE gene family's functions in plants. Additionally, a KNOX-BELL protein interaction network in chrysanthemum was constructed, revealing 31 interaction pairs, including seven previously unreported combinations. The study also finds that the overexpression of CmBLH2 enhanced the activity of antioxidant system, reducing cellular damage under cold stress, while RNAi lines exhibited lower reactive oxygen species scavenging capacity. This research lays the foundation for further investigation of the TALE gene family's roles in development and stress responses in chrysanthemum and other species.

Keywords: Chrysanthemum morifolium; Cold stress; Expression pattern; Protein-protein interaction; TALE transcription factor family.