Immune correlates of early clearance of Mycobacterium tuberculosis among tuberculosis household contacts in Indonesia

Nat Commun. 2025 Jan 2;16(1):309. doi: 10.1038/s41467-024-55501-6.

Abstract

Some individuals, even when heavily exposed to an infectious tuberculosis patient, do not develop a specific T-cell response as measured by interferon-gamma release assay (IGRA). This could be explained by an IFN-γ-independent adaptive immune response, or an effective innate host response clearing Mycobacterium tuberculosis (Mtb) without adaptive immunity. In heavily exposed Indonesian tuberculosis household contacts (n = 1347), a persistently IGRA negative status was associated with presence of a BCG scar, and - especially among those with a BCG scar - with altered innate immune cells dynamics, higher heterologous (Escherichia coli-induced) proinflammatory cytokine production, and higher inflammatory proteins in the IGRA mitogen tube. Neither circulating concentrations of Mtb-specific antibodies nor functional antibody activity associated with IGRA status at baseline or follow-up. In a cohort of adults in a low tuberculosis incidence setting, BCG vaccination induced heterologous innate cytokine production, but only marginally affected Mtb-specific antibody profiles. Our findings suggest that a more efficient host innate immune response, rather than a humoral response, mediates early clearance of Mtb. The protective effect of BCG vaccination against Mtb infection may be linked to innate immune priming, also termed 'trained immunity'.

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • BCG Vaccine* / administration & dosage
  • BCG Vaccine* / immunology
  • Cytokines / blood
  • Cytokines / immunology
  • Cytokines / metabolism
  • Family Characteristics
  • Female
  • Humans
  • Immunity, Innate* / immunology
  • Indonesia / epidemiology
  • Interferon-gamma / immunology
  • Interferon-gamma Release Tests
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis* / immunology
  • Tuberculosis* / epidemiology
  • Tuberculosis* / immunology
  • Tuberculosis* / microbiology
  • Young Adult

Substances

  • BCG Vaccine
  • Cytokines
  • Interferon-gamma
  • Antibodies, Bacterial