Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle

Pingping Cai; Christian J Casas; Gabriel Quintero Plancarte; Takashi Mikawa; Lisa L Hua

doi:10.1007/s10577-024-09760-0

Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle

Chromosome Res. 2025 Jan 3;33(1):1. doi: 10.1007/s10577-024-09760-0.

Authors

Pingping Cai^#¹, Christian J Casas^#², Gabriel Quintero Plancarte^#², Takashi Mikawa³, Lisa L Hua⁴

Affiliations

¹ Cardiovascular Research Institute, University of California, San Francisco, CA, USA.
² Department of Biology, Sonoma State University, Rohnert Park, CA, USA.
³ Cardiovascular Research Institute, University of California, San Francisco, CA, USA. [email protected].
⁴ Department of Biology, Sonoma State University, Rohnert Park, CA, USA. [email protected].

^# Contributed equally.

Abstract

Little is known about how distance between homologous chromosomes are controlled during the cell cycle. Here, we show that the distribution of centromere components display two discrete clusters placed to either side of the centrosome and apical/basal axis from prophase to G₁ interphase. 4-Dimensional live cell imaging analysis of centromere and centrosome tracking reveals that centromeres oscillate largely within one cluster, but do not cross over to the other cluster. We propose a model of an axis-dependent ipsilateral restriction of chromosome oscillations throughout mitosis.

Keywords: Cell cycle; Centromeres; Chromosomes; Homologous chromosomes; Mitosis.

MeSH terms

Cell Cycle*
Centromere* / genetics
Centrosome* / metabolism
Chromosomes
Humans
Interphase
Mitosis

Abstract

MeSH terms

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