Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone

Wan-Zheng Fang; Sathyadevi Palanisamy; Priya Vijayaraghavan; Chiao-Yun Chen; Shun-Long Weng; Chung-Shi Yang; Yun-Ming Wang

doi:10.1016/j.bios.2024.117113

Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone

Biosens Bioelectron. 2024 Dec 30:272:117113. doi: 10.1016/j.bios.2024.117113. Online ahead of print.

Authors

Wan-Zheng Fang¹, Sathyadevi Palanisamy², Priya Vijayaraghavan¹, Chiao-Yun Chen³, Shun-Long Weng⁴, Chung-Shi Yang⁵, Yun-Ming Wang⁶

Affiliations

¹ Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), Yang Ming Chiao Tung University, Hsinchu 300, Taiwan.
² Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), Yang Ming Chiao Tung University, Hsinchu 300, Taiwan; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
³ Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan; School of Post-Baccalaureate Medicine, College of Medicine, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: [email protected].
⁴ Department of Medicine, MacKay Medical College, New Taipei City 252, Taiwan; Department of Obstetrics and Gynecology, Hsinchu MacKay Memorial Hospital, Hsinchu 300, Taiwan; Department of Obstetrics and Gynecology, Hsinchu Municipal MacKay Children's Hospital, Hsinchu 300, Taiwan. Electronic address: [email protected].
⁵ Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Zhunan 350, Taiwan. Electronic address: [email protected].
⁶ Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), Yang Ming Chiao Tung University, Hsinchu 300, Taiwan; Department of Biomedical Science and Environmental Biology, School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: [email protected].

PMID: 39754847
DOI: 10.1016/j.bios.2024.117113

Abstract

Anti-mullerian hormone (AMH) detection receives much attention since it is used as an ideal biomarker for quantitative assessment of ovarian reserve. The present study proposed a first report on the use of MOF-on-MOF as an electrochemical sensor for recognizing AMH in buffer and serum media. The MOF-on-MOF, MIL-88 B@UiO66NH₂ was synthesized by the internal extended growth method (IEGM) involving MIL-88 B on UiO66NH₂ by in situ method for the first time. MOF matrix could be established to form a three-dimensional (3D) core-shell hybrid unit using MOFs with distinct characteristics. The morphology, structural characteristics, and electrochemical performance of MIL-88 B@UiO66NH₂ were studied. It was successfully used for AMH sensing to demonstrate the detection performance of the internal extended growth method (IEGM) grown MIL-88 B@UiO66NH₂ made immunosensor. The electrochemical results indicated that MOF-on-MOF exhibited linear EIS response for AMH concentration varying from 100 ng/mL to 1 fg/mL. Further, the immunosensor displayed high specificity and sensitivity for AMH detection. The fabricated sensor attained a remarkable limit of detection (LOD) of 1.07 fg/mL and 0.82 fg/mL, when studied in PBS and 10% serum buffer media, respectively. The biosensor achieved the limit of quantification (LOQ) of 3.25 fg/mL and 2.5 fg/mL, respectively, when analyzed in PBS buffer and 10% serum buffer. The significant results emphasized that the fabricated biosensor holds a promising potential to act as an appropriate tool for rapid assessment of AMH levels.

Keywords: Anti-mullerian hormone; Biomarker; Device; MOF-On-MOF; Sensing; Serum.