Concrete, as the most widely used construction material globally, is prone to cracking under the influence of external factors such as mechanical loads, temperature fluctuations, chemical corrosion, and freeze-thaw cycles. Traditional concrete crack repair methods, such as epoxy resins and polymer mortars, often suffer from a limited permeability, poor compatibility with substrates, and insufficient long-term durability. Microbial biogrouting technology, leveraging microbial-induced calcium carbonate precipitation (MICP), has emerged as a promising alternative for crack sealing. This study aimed to explore the potential of Bacillus pasteurii for repairing concrete cracks to enhance compressive strength and permeability performance post-repair. Experiments were conducted to evaluate the bacterial growth cycle and urease activity under varying concentrations of Ca2+. The results indicated that the optimal time for crack repair occurred 24-36 h after bacterial cultivation. Additionally, the study revealed an inhibitory effect of high calcium ion concentrations on urease activity, with the optimal concentration identified as 1 mol/L. Compressive strength and water absorption tests were performed on repaired concrete specimens. The compressive strength of specimens with cracks of varying dimensions improved by 4.01-11.4% post-repair, with the highest improvement observed for specimens with 1 mm wide and 10 mm deep cracks, reaching an increase of 11.4%. In the water absorption tests conducted over 24 h, the average mass water absorption rate decreased by 31.36% for specimens with 0.5 mm cracks, 29.06% for 1 mm cracks, 27.9% for 2 mm cracks, and 28.2% for 3 mm cracks. X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses confirmed the formation of dense calcium carbonate precipitates, with the SEM-EDS results identifying calcite and vaterite as the predominant self-healing products. This study underscores the potential of MICP-based microbial biogrouting as a sustainable and effective solution for enhancing the mechanical and durability properties of repaired concrete.
Keywords: biogrout; concrete; cracking; mechanical properties; microbial-induced carbonate precipitation (MICP); water resistance.