Inosine monophosphate dehydrogenase 2 (IMPDH2) modulates response to therapy and chemo-resistance in triple negative breast cancer

Sci Rep. 2025 Jan 7;15(1):1061. doi: 10.1038/s41598-024-85094-5.

Abstract

Triple negative breast cancer (TNBC) is one of the deadliest subtypes of breast cancer, whose high frequency of relapse is often due to resistance to chemotherapy. Here, we identify inosine monophosphate dehydrogenase 2 (IMPDH2) as a contributor to doxorubicin resistance, in multiple TNBC models. Analysis of publicly available datasets reveals elevated IMPDH2 expression to associate with worse overall TNBC prognosis in the clinic, including lower recurrence-free survival post adjuvant/neoadjuvant therapy. Importantly, both genetic depletion and pharmacological inhibition of IMPDH2 leads to reduction of pro-tumorigenic phenotypes in multiple doxorubicin-resistant TNBC models, both in vitro and in vivo. Overall, we propose IMPDH2 as a novel vulnerability that could be leveraged therapeutically to suppress and/or prevent the growth of chemo-resistant lesions.

Keywords: Chemo-resistance; Doxorubicin; GTP metabolism; IMPDH2; Paclitaxel; Ribavirin; TNBC.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Doxorubicin* / pharmacology
  • Doxorubicin* / therapeutic use
  • Drug Resistance, Neoplasm* / genetics
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • IMP Dehydrogenase* / antagonists & inhibitors
  • IMP Dehydrogenase* / genetics
  • IMP Dehydrogenase* / metabolism
  • Mice
  • Prognosis
  • Triple Negative Breast Neoplasms* / drug therapy
  • Triple Negative Breast Neoplasms* / genetics
  • Triple Negative Breast Neoplasms* / metabolism
  • Triple Negative Breast Neoplasms* / pathology
  • Xenograft Model Antitumor Assays

Substances

  • IMP Dehydrogenase
  • IMPDH2 protein, human
  • Doxorubicin