Diselenides as novel effective fluorescence quenchers to construct a two-photon fluorescent probe for thiols in a mouse stroke model

Tao Ma; Hao Gao; Jun Wu; Jintao Zhao; Bingbing Chang; Zhibin Zhang; Shengxiang Zhang; Baoxin Zhang; Jianguo Fang

doi:10.1039/d4cc06286h

Diselenides as novel effective fluorescence quenchers to construct a two-photon fluorescent probe for thiols in a mouse stroke model

Chem Commun (Camb). 2025 Jan 8. doi: 10.1039/d4cc06286h. Online ahead of print.

Authors

Tao Ma¹, Hao Gao², Jun Wu¹, Jintao Zhao¹, Bingbing Chang¹, Zhibin Zhang¹, Shengxiang Zhang², Baoxin Zhang¹, Jianguo Fang^{1

3}

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. [email protected].
² Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China. [email protected].
³ School of Chemistry and Chemical Engineering, Nanjing University of Science & Technology, Nanjing, Jiangsu 210094, China.

PMID: 39774747
DOI: 10.1039/d4cc06286h

Abstract

A fluorescence quenching mechanism using linear diselenides was proposed for the first time through a combination of intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET). Herein, we synthesized and screened a two-photon fluorescent probe AFC-SeSe, demonstrating a remarkable 300-fold increase in response to glutathione (GSH). Additionally, AFC-SeSe enabled real-time observation of increased thiol levels following treatment within a short timeframe in a mouse model of stroke.