Cyvirus cyprinidallo 2 (CyHV-2) is the pathogen of herpesviral hematopoietic necrosis (HVHN) that mainly infects goldfish Carassius auratus and crucian carp C. carassius and is characterized by high infectivity and pathogenicity. The availability of rapid and convenient detection methods is essential for early detection of CyHV-2. A colloidal gold immunochromatographic strip using 2 specific anti-CyHV-2 monoclonal antibodies (MAbs) has been developed and validated for rapid detection of CyHV-2. The test results can be viewed within 10min. The detection limit of test strip was 2.08 × 102TCID50 ml-1, and it showed no cross-reactivity with other freshwater fish viral pathogen except KHV (koi herpesvirus). The specificity of the strip was 100% when spleen and kidney tissues of CyHV-2 infected and healthy crucian carp were assayed following an experimental challenge. The colloidal gold immunochromatographic strip will be an effective device for the rapid detection of CyHV-2 in the future.
Keywords: Colloidal gold immunochromatography assay; CyHV-2; GICA; Monoclonal antibodies; Rapid detection.